Methods
Methods
We have expertise in the following methods, among others:
in vitro-Models
- various human cell lines
Liver (HepG2), Lung (A549), Intestine/colon (Caco-2, HT-29-MTX, LS174T), Bladder (UROtsa), Brain (Astrocytes: CCF-STTG, Neurones: Luhmes), Fibroblasts (VH10-hTert), human Monocytes (THP-1) - 3D-Models for the intetestinal barrier incl. mucus layer
Caco-2, Caco-2/HT-29-MTX
Testsystems
- Cytotoxicity assays
(Cellcount, celldeath (PI, DAPI, calcein-AM), colonyformingability, lysosomal integrity, mitochondrial activity, dehydrogenase activity, proteinconcentration, necrosis (LDH), apoptosis...) - Cellular stress
(mitochondrial membrane potential, RONS, superoxides ...) - Genotoxicity tests
(Micronucleustest, alkalinedebinding, γH2AX-Foci, COMET Assay (Comet Assay IVTM)...) - Western Blot, qPCR, SlotBlot, Protein activity of selected DNA-Rep-Proteins
Transport Studies
- Cellular bioavailability
- In vitro gastrointestinal digestion model
- Barrier integrity (CellZscope®, IHC analysis der tight junction proteins...)
Mucin- und Glycan analytics:
- Lectin-blots
- Histochemical and immunochemical Mukusdetection and imaging in cells and tissue (AB, PAS, Lectins, StcE, MUC2,…)
- Glycogene- und Glycoproteinexpression
Element- und Metal analytics:
- Multi-element method for the detection of micronutrients in biosamples (food, tissue, cells, serum)
- Analysis of toxic heavy metals in biosamples
- Low molecular weight metal-responsive fluorescence probes for quantitative or imaging analysis of labile (sub)cellular metal species in cells and tissue
Instruments
- ICP-MS/MS (Agilent 8800)
(Metal quantification, metal speciation with LC-ICP-MS/MS-Coupling,...) - Atom absorption spektrometry (Perkin Elmer 600 Analyst)
- LC-MS/MS (Agilent 6595)
(amino acids, GSH/GSSG, …) - HPLC-DAD/FD (Agilent, Shimadzu, ...)
- Fluorescence-Mikroskopes (Leica)
- Mikrowave-assisted digestion system (CEM)
- MALDI-TOF MS (Bruker)