Range of methods

Methods

The department has the expertise for the following list of methods (selection):

Protein analytics

  • Colorimetric protein quantification (e.g. Bradford, BCA)
  • ELISA for specific protein quantification: in-house development and validation, e.g. Gc-globulin, retinol binding protein 4, transthyretin, Tamm-Horsfall protein
  • 1D- and 2D-polyacrylamid gel electrophoresis with subsequent visualization of proteins by immunoblotting, silver nitrate or coomassie staining
  • Dot-Blot
  • Protein identification by enzymatic digestion (in-gel-digestions, in-solution-digestion) and MALDI-TOF-MS
  • Characterization of protein modifications by MALDI-TOF-MS (e.g. subsequent to immunoprecipitation)

Lipids and lipophilic substances

  • HPLC: Quantification of carotenoids, retinol, retinyl esters and vitamin E metabolites
  • LC-MS/MS: Quantification of vitamin D metabolites
  • Thinlayer chromatographie of neutral and phospholipids
  • Ultracentrifugation for the separation of lipoproteins

Aminothiol analysis

  •  HPLC

Clinical chemistry

  • Creatinine
  • Urea
  • Glucose
  • Insulin
  • Triglycerids
  • Cholesterol
  • Antioxidative capacity (FRAP assay)

Hematology

  • Erythrocyte count
  • Hematocrite
  • Hemoglobin
  • Blood sedimentation speed
  • Blood typing
  • Leukocyte count
  • Differential blood count

Histology

  • Conventional histology
  • Immunohistochemistry

Cell culture

Large equipment

The department is equipped with the following of large-scale devices (selection):

  • HPLC with PDA and fluorescence detector (Shimadzu, Waters)
  • HPLC – Infinity 1290 (Agilent)
  • Triple quadrupole mass spectrometer – Agilent 6460; ionization sources: ESI, APCI (Agilent)
  • MALDI-TOF-MS – Autoflex (Bruker)
  • LC-MALDI Fraction Collector (Bruker)
  • ImagePrep (Bruker)
  • Surface plasmon resonance spectrometer  (Biacore)
  • Accelerated Solvent Extraction (Dionex)
  • Ultracentrifuge - Ultra Pro 80 (Sorvall)