Time-resolved crystallography reveals water mediated allostery as new mechanism for information transfer in proteins. In a series of experiment, carried out at PETRA III Hamburg, we have observed the full catalytic reaction mechanism in the enzyme defluorinase and found a novel mechanism for allosteric long range communication.
The paper is the result of a collaboration between U Toronto, MPSD Hamburg and U Potsdam.
Mehrabi, P. et al - Time-resolved crystallography reveals allosteric communication aligned with molecular breathing, Science 13 Sep 2019: Vol. 365, Issue 6458, pp. 1167-1170 DOI: 10.1126/science.aaw9904
Finally - the idea for this review dates back a while to some very fruitful conference discussions among the three of us authors, who are all very active in method development for serial crystallography experiments. We originally planned to mainly write about fixed targets for serial crystallography, now this turned into a full review of all currently available approaches on "how to get your sample into the beam" if you want to do a serial X-Ray experiment . The methods are available and used broadly now, both at XFELs and for the newly emerging technique of serial synchrotron crystallography.
The paper is published here: Martiel, I., Müller-Werkmeister, H. M., Cohen, A. E., Strategies for sample delivery for femtosecond crystallography, Acta Cryst. (2019). D75, 160-177 https://doi.org/10.1107/S2059798318017953
The first results on photocaged compounds from our great collaboration with Arwen Pearson's group at Universty of Hamburg are published. Great synthesis Joe!... and Yannik, congratulations to get part of your Master thesis out.
We contributed by laser flash photolysis experiments, quite challenging as the sample needed to be replaced for every single scan (...the difficulty of characterising irreversible reactions).
Photocaged compounds are promising tools for applications like time-resolved crystallography, as they allow to initiate reaction dynamics by light to release a substrate (i.e. the substrate for an enzyme) or activate a biological process (i.e. by uncaging a residue in an active center).
Find the paper here: https://pubs.rsc.org/en/content/articlehtml/2019/ra/c9ra00968j
HARE- SSX, Hit-and-return serial synchrotron crystallography is a new technical approach that allows the study of protein dynamics from milliseconds to seconds. We demonstrate here the principle, which is based on using the crystallography chip developed at U Toronto and MPSD Hamburg.
The paper can be found here: https://www.nature.com/articles/s41592-018-0180-2
Talk at ICUSD Trieste. Rike is presenting the recent UED results at the fourth international conference on ultrafast structural dynamics.
Rike was interviewed for the Potsdamer Neue Nachrichten - as one of the new professors this year with an emphasis on her research and the fact that she is hired within the Potsdamer Model for tenure track positions in Germany. Thanks to Silke Engel for this great article and off to a great start in Potsdam. Read here the full version in German: "In die Zellen zoomen" (on page 2)