Biomolecular Interaction Analysis with heliX+
Understanding how biomolecules behave is central to medical research, for example in the development of new vaccines or assays for the quick and sensitive diagnostic of diseases. Biomolecular interaction aims to determine and characterise how biomolecules interact with each other.
In the project “BIOMIA-Dx”, we aim to analyse biomolecular interaction of different diagnostic targets as well as characterising molecular methods in real time. For this purpose, we use the helix+ and ProFIRE Systems (Dynamic Biosensors, Munich, Germany).
Our aims include interaction analysis for bioanalytic targets as well as the development of isothermal amplification methods:
- Kinetic constants of hybridisation between short oligomers (primers and probes) to immobilised templates
- Binding analysis of aptamers to Aflatoxin
- Analysis of binding constants and affinity from proteins, protein domains and SARS-CoV-2 peptides to hACE2 receptor
- Analysis of binding constants and affinity from proteins, protein domains and antibody-derived peptides to the Spike protein of SARS-CoV-2
- Binding analysis of DNA-binding proteins
- Analysis of the binding and synthesis activity of polymerases