Range of methods
Methods
The department has the expertise for the following list of methods (selection):
Protein analytics
- Colorimetric protein quantification (e.g. Bradford, BCA)
- ELISA for specific protein quantification: in-house development and validation, e.g. Gc-globulin, retinol binding protein 4, transthyretin, Tamm-Horsfall protein
- 1D- and 2D-polyacrylamid gel electrophoresis with subsequent visualization of proteins by immunoblotting, silver nitrate or coomassie staining
- Dot-Blot
- Protein identification by enzymatic digestion (in-gel-digestions, in-solution-digestion) and MALDI-TOF-MS
- Characterization of protein modifications by MALDI-TOF-MS (e.g. subsequent to immunoprecipitation)
Lipids and lipophilic substances
- HPLC: Quantification of carotenoids, retinol, retinyl esters and vitamin E metabolites
- LC-MS/MS: Quantification of vitamin D metabolites
- Thinlayer chromatographie of neutral and phospholipids
- Ultracentrifugation for the separation of lipoproteins
Aminothiol analysis
- HPLC
Clinical chemistry
- Creatinine
- Urea
- Glucose
- Insulin
- Triglycerids
- Cholesterol
- Antioxidative capacity (FRAP assay)
Hematology
- Erythrocyte count
- Hematocrite
- Hemoglobin
- Blood sedimentation speed
- Blood typing
- Leukocyte count
- Differential blood count
Histology
- Conventional histology
- Immunohistochemistry
Cell culture
Large equipment
The department is equipped with the following of large-scale devices (selection):
- HPLC with PDA and fluorescence detector (Shimadzu, Waters)
- HPLC – Infinity 1290 (Agilent)
- Triple quadrupole mass spectrometer – Agilent 6460; ionization sources: ESI, APCI (Agilent)
- MALDI-TOF-MS – Autoflex (Bruker)
- LC-MALDI Fraction Collector (Bruker)
- ImagePrep (Bruker)
- Surface plasmon resonance spectrometer (Biacore)
- Accelerated Solvent Extraction (Dionex)
- Ultracentrifuge - Ultra Pro 80 (Sorvall)