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Publikation der Abteilung Biochemie der Ernährung


Neuschäfer-Rube F, Schön T, Kahnt I, Püschel GP. LDL-Dependent Regulation of TNFα/PGE2 Induced COX-2/mPGES-1 Expression in Human Macrophage Cell Lines. Inflammation. 2023 Jan 4. doi: 10.1007/s10753-022-01778-y. Online ahead of print. PMID: 36598592

Schjeide BM, Püschel GP. Determining On-Target, Off-Target, and Copy Number Status of Transgenic Events After CRISPR/Cas9 Targeted AAVS1 Safe-Harbor Modification of iPSCs Using Double-Control Quantitative Copy Number PCR. Curr Protoc. 2023 Jan;3(1):e635. doi: 10.1002/cpz1.635. PMID: 36598341


Püschel GP, Klauder J, Henkel J. Macrophages, Low-Grade Inflammation, Insulin Resistance and Hyperinsulinemia: A Mutual Ambiguous Relationship in the Development of Metabolic Diseases. J Clin Med. 2022 Jul 27;11(15):4358. doi: 10.3390/jcm11154358. PMID: 35955975

Schäfer M, Kakularam KR, Reisch F, Rothe M, Stehling S, Heydeck D, Püschel GP, Kuhn H. Male Knock-in Mice Expressing an Arachidonic Acid Lipoxygenase 15B (Alox15B) with Humanized Reaction Specificity Are Prematurely Growth Arrested When Aging. Biomedicines. 2022 Jun 10;10(6):1379. doi: 10.3390/biomedicines10061379. PMID: 35740398

Schjeide BM, Schenke M, Seeger B, Püschel GP. Validation of a Novel Double Control Quantitative Copy Number PCR Method to Quantify Off-Target Transgene Integration after CRISPR-Induced DNA Modification.  Methods Protoc. 2022 May 25;5(3):43. doi: 10.3390/mps5030043. PMID: 3573654

Heydeck D, Reisch F, Schäfer M, Kakularam KR, Roigas SA, Stehling S,Püschel GP, Kuhn H. The Reaction Specificity of Mammalian ALOX15 Orthologs is Changed During Late Primate Evolution and These Alterations Might Offer Evolutionary Advantages for Hominidae. Front Cell Dev Biol. 2022 Apr 21;10:871585. doi: 10.3389/fcell.2022.871585. eCollection 2022. PMID: 35531094

Neuschäfer-Rube F, Pathe-Neuschäfer-Rube A, Püschel GP. Discrimination of the Activity of Low-Affinity Wild-Type and High-Affinity Mutant Recombinant BoNT/B by a SIMA Cell-Based Reporter Release Assay.  Toxins (Basel). 2022 Jan 17;14(1):65. doi: 10.3390/toxins14010065. PMID: 35051041


Henkel J, Klauder J, Statz M, Wohlenberg AS, Kuipers S, Vahrenbrink M, Püschel GP. Enhanced Palmitate-Induced Interleukin-8 Formation in Human Macrophages by Insulin or Prostaglandin E2. Biomedicines. 2021 Apr 21;9(5):449. doi: 10.3390/biomedicines9050449. PMID: 33919366

Pathe-Neuschäfer-Rube A, Neuschäfer-Rube F, Püschel GP.Cell-Based Reporter Release Assay to Determine the Activity of Calcium-Dependent Neurotoxins and Neuroactive Pharmaceuticals.Toxins (Basel). 2021 Mar 30;13(4):247. doi: 10.3390/toxins13040247. PMID: 33808507


Klauder J, Henkel J, Vahrenbrink M, Wohlenberg AS, Camargo RG, Püschel GP. Direct and indirect modulation of LPS-induced cytokine production by insulin in human macrophages Cytokine. 2020 Dec;136:155241. doi: 10.1016/j.cyto.2020.155241. Epub 2020 Aug 13. PMID: 32799102

Gehre C, Flechner M, Kammerer S, Küpper JH, Coleman CD, Püschel GP, Uhlig K, Duschl C. Real time monitoring of oxygen uptake of hepatocytes in a microreactor using optical microsensors. Sci Rep. 2020 Aug 13;10(1):13700. doi: 10.1038/s41598-020-70785-6.

Schell M, Chudoba C, Leboucher A, Alfine E, Flore T, Ritter K, Weiper K, Wernitz A, Henkel J, Kleinridders A. Interplay of Dietary Fatty Acids and Cholesterol Impacts Brain Mitochondria and Insulin Action. Nutrients. 2020 May 23;12(5):1518. doi: 10.3390/nu12051518.

Schenke M, Schjeide BM, Püschel GP, Seeger B. Analysis of Motor Neurons Differentiated from Human Induced Pluripotent Stem Cells for the Use in Cell-Based Botulinum Neurotoxin Activity Assays. Toxins (Basel). 2020 Apr 25;12(5):276. doi: 10.3390/toxins12050276.


Henkel J, Buchheim-Dieckow K, Castro JP, Laeger T, Wardelmann K, Kleinridders A, Jöhrens K, Püschel GP. Reduced Oxidative Stress and Enhanced FGF21 Formation in Livers of Endurance-Exercised Rats with Diet-Induced NASH. Nutrients. 2019 Nov 8;11(11)

Püschel GP, Henkel J. Dietary cholesterol does not break your heart but kills your liver. Porto Biomed J. 2019 Jun 29;3(1):e12.


Henkel J, Coleman CD, Schraplau A, Jöhrens K, Weiss TS, Jonas W, Schürmann A, Püschel GP. Augmented liver inflammation in a microsomal prostaglandin E synthase 1 (mPGES-1)-deficient diet-induced mouse NASH model. Sci Rep . 2018 Oct 31;8(1):16127

Henkel J, Alfine E, Saín J, Jöhrens K, Weber D, Castro JP, König J, Stuhlmann C, Vahrenbrink M, Jonas W, Kleinridders A, Püschel GP. Soybean Oil-Derived Poly-Unsaturated Fatty Acids Enhance Liver Damage in NAFLD Induced by Dietary Cholesterol. Nutrients . 2018 Sep 18;10(9)

Pathe-Neuschäfer-Rube A, Neuschäfer-Rube F, Haas G, Langoth-Fehringer N, Püschel GP. Cell-Based Reporter Release Assay to Determine the Potency of Proteolytic Bacterial Neurotoxins. Toxins (Basel). 2018 Sep 5;10(9)

Neuschäfer-Rube F, Pathe-Neuschäfer-Rube A, Hippenstiel S, Püschel GP. PGE 2 enhanced TNFa-mediated IL-8 induction in monocytic cell lines and PBMC. Cytokine . 2018 Jun 18(18)30271-0

Knebel C, Neeb J, Zahn E, Schmidt F, Carazo A, Holas O, Pavek P, Püschel GP, Zanger UM, Süssmuth R, Lampen A, Marx-Stoelting P, Braeuning A. (2018) Unexpected Effects of Propiconazole, Tebuconazole, and Their Mixture on the Receptors CAR and PXR in Human Liver Cells. Toxicol Sci . 2018 May 1;163(1):170-181


von Loeffelholz C, Lieske S, Neuschäfer-Rube F, Willmes DM, Raschzok N, Sauer IM, König J, Fromm MF, Horn P, Chatzigeorgiou A, Pathe-Neuschäfer-Rube A, Jordan J, Pfeiffer AFH, Mingrone G, Bornstein SR, Stroehle P, Harms C, Wunderlich FT, Helfand SL, Bernier M, de Cabo R, Shulman GI, Chavakis T, Püschel GP, Birkenfeld AL. (2017) Hepatology 2017 J30

Henkel J, Coleman CD, Schraplau A, J?hrens K, Weber D, Castro JP, Hugo M, Schulz TJ, Krämer S, Schürmann A, Püschel GP. (2017) Induction of steatohepatitis (NASH) with insulin resistance in wildtype B6 mice by a western-type diet containing soybean oil and cholesterol. Mol Med . 2017 21;23


Hocher B, Haumann H, Rahnenführer J, Reichetzeder C, Kalk P, Pfab T, Tsuprykov O, Winter S, Hofmann U, Li J, Püschel GP, Lang F, Schuppan D, Schwab M, Schaeffeler E.(2016) Maternal eNOS deficiency determines a fatty liver phenotype of the offspring in a sex dependent manner. Epigenetics . 2016 11:539-552

Manowsky J, Camargo RG, Kipp AP, Henkel J, Püschel GP.(2016) Insulin-induced cytokine production in macrophages causes insulin resistance in hepatocytes. Am J Physiol Endocrinol Metab. 2016 310:938-946


Seelaender M, Laviano A, Busquets S, Püschel GP, Margaria T, Batista ML Jr. (2015) Inflammation in Chachexia Mediators Inflamm . 2015

Pathe-Neuschäfer-Rube A, Neuschäfer-Rube F, Genz L and Püschel GP (2015) Botulinum Neurotoxin Dose-Dependently Inhibits Release of Neurosecretory Vesicle-Targeted Luciferase from Neuronal Cells . Altex. 2015 32:297-306

Neuschäfer-Rube F, Schraplau A, Schewe B, Lieske S, Krützfeld J-M, Ringel S, Henkel J, Birkenfeld A and Püschel GP (2015) Arylhydrocarbon receptor-dependent mIndy (Slc13a5) induction as possible contributor to benzo[a]pyrene-induced lipid accumulation in hepatocytes. Toxicology. 2015 337:1-9

Schraplau A, Schewe B, Neuschäfer-Rube F, Ringel S, Neuber C, Kleuser B, Püschel GP (2015) Enhanced thyroid hormone breakdown in hepatocytes by mutual induction of the constitutive androstane receptor (CAR, NR1I3) and arylhydrocarbon receptor by benzo[a]pyrene and phenobarbital. Toxicology 2015 328:21-28


Neuschäfer-Rube F, Lieske S, Kuna M, Henkel J, Perry R, Erion D, Pesta D, von Loeffelholz C, Willmes D, Tolkachov A, Brachs S, Schupp M, Pfeiffer A, Pathe-Neuschäfer-Rube A, Shulman G, Püschel GP and Birkenfeld, A (2014) The Mammalian INDY Homolog is Induced by CREB in a Rat Model of Type 2 Diabetes Diabetes. 2014 63:1048-5

Fayyaz S, Henkel J, Japtok L, Krämer S, Damm G, Seehofer D, Püschel GP, Kleuser B (2014) Involvement of sphingosine 1-phosphate in palmitate-induced insulin resistance of hepatocytes via the S1P2 receptor subtype. Diabetologia . 2014 57:373-82


Neuschäfer-Rube F, Pathe-Neuschäfer-Rube A, Hippenstiel S, Kracht M, Püschel GP (2013) NF-KappaB-dependent IL-8 induction by prostaglandin E2 receptors EP1 and EP4. Br J Pharmacol . 2013 168:704-717


Henkel J, Frede K, Schanze N, Vogel H, Schürmann A, Spruss A, Bergheim I, Püschel GP. (2012) Stimulation of fat accumulation in hepatocytes by PGE(2)-dependent repression of hepatic lipolysis, ß-oxidation and VLDL-synthesis. Lab Invest. 2012 92:1597-606.

Spruss A, Henkel J, Kanuri G, Blank D, Püschel GP, Bischoff SC, Bergheim I (2012) Female mice are more susceptible to non-alcoholic fatty liver disease: sex-specific regulation of the hepatic AMP-activated protein kinase - plasminogen activator inhibitor 1-cascade but not the hepatic endotoxin response Molmed 2012 18:1346-1355

Hesse D, Jaschke A, Kanzleiter T, Witte N, Augustin R, Hommel A, Püschel GP, Petzke KJ, Joost HG, Schupp M, Schürmann A (2012) GTPase ARFRP1 is essential for normal hepatic glycogen storage and insulin-like growth factor 1 secretion. Mol Cell Biol .2012 32:4363-74

Szymanski KV, Toennies M, Becher A, Fatykhova D, N'guessan PD, Gutbier B, Klauschen F, Neuschaefer-Rube F, Schneider P, Rueckert J, Neudecker J, Bauer TT, Dalhoff K, Drömann D, Gruber AD, Kershaw O, Temmesfeld-Wollbrueck B, Suttorp N, Hippenstiel S, Hocke AC. (2012) Streptococcus pneumoniae-induced regulation of cyclooxygenase-2 in human lung tissue. Eur Respir J . 2012 40(6):1458-67


Henkel J, Gärtner D, Dorn C, Hellerbrand C, Schanze N, Elz SR, Püschel GP. (2011) Oncostatin M produced in Kupffer cells in response to PGE2: possible contributor to hepatic insulin resistance and steatosis Lab Invest . 2011 91:1107-17


Wieneke N , Neuschäfer-Rube F , Bode LM , Kuna M , Andres J , Carnevali LC Jr , Hirsch-Ernst KI , Püschel GP. (2009) Synergistic acceleration of thyroid hormone degradation by phenobarbital and the PPAR alpha agonist WY14643 in rat hepatocytes. Toxicol Appl Pharmacol. 2009 240;99-107

Henkel J, Neuschäfer-Rube F, Pathe-Neuschäfer-Rube A, Püschel GP (2009) Aggravation by prostaglandin E2 of interleukin-6-dependent insulin resistance in hepatocytes. Hepatology. 2009 50;781-90


Wieneke N, Hirsch-Ernst KI, Kuna M, Kersten S, Püschel GP (2008) PARalpha-dependent induction of the energy homeostasis-regulating nuclear receptor NR1i3 (CAR) in rat hepatocytes: potential role in starvation adaptation. FEBS Lett. 2007 581;5617-26.


Giening G, Jurgensen J, Eichhorst J, Furkert J, Beyermann M, Neuschafer-Rube F, Rosenthal W, Zidek W, van der Giet M and Oksche A (2005) Arrestin-Independent Internalization and Recycling of the Urotensin Receptor Contribute to Long-Lasting Urotensin II-Mediated Vasoconstriction.  Circ Res. 2005 Sep 30;707-15.

Neuschäfer-Rube F, Hermosilla R, Kuna M, Pathe-Neuschäfer-Rube A, Schülein R, Puschel GP. (2005) A Ser/Thr cluster within the C-terminal domain of the rat prostaglandin receptor EP3alpha is essential for agonist-induced phosphorylation, desensitization and internalization. Br J Pharmacol. 2005 Aug 145;1132-42

Pathe-Neuschäfer-Rube A, Neuschäfer-Rube F and Püschel GP. (2005) Role of the ERC-motif in the proximal part of the second intracellular loop and the C-terminal domain of the human prostaglandin F2alpha receptor (FP receptor) in G-protein coupling control. Biochem J. 2005 Jan14; 388:317-324


Püschel GP. (2004) Control of hepatocyte metabolism by sympathetic and parasympathetic hepatic nerves.  Anat Rec. 280A:854-867.

Neuschäfer-Rube F, Hermosilla R, Rehwald M, Rönnstrand L, Schülein R, Wernstedt C, Püschel GP. (2004) Identification of a Ser/Thr cluster in the C-terminal domain of the human prostaglandin receptor EP4 that is essential for agonist-induced beta-arrestin1 recruitment but differs from the apparent principal phosphorylation site. Biochem J. 379:573-85


Boer U, Fennekohl A, Püschel GP. (2003) Sensitization by interleukin-6 of rat hepatocytes to tumor necrosis factor alpha-induced apoptosis. J Hepatol. 38:728-35

Neuschäfer-Rube F, Engemaier E, Koch S, Boer U, Püschel GP. (2003) Identification by site-directed mutagenesis of amino acids contributing to ligand-binding specificity or signal transduction properties of the human FP prostanoid receptor. Biochem J. 371:443-9