Available methods

CD spectroscopy

Structure determination of chiral (bio)molecules and investigation of their interactions with other molecules

EPR spectroscopy

Electron spin resonance measurements of paramagnetic substances, including under irradiation

Fluorescence spectroscopy

Fluorimetric measurements

Fluorescence quantum yield

Determination of the quantum yield in the range 300-950 nm

IR spectroscopy

Recording of IR spectra in transmission using KBr and CsI pellets (4000–500 cm⁻¹ and 1000–80 cm⁻¹, respectively) or by attenuated total reflection (ATR) using a diamond or ZnSe crystal (30 000–200 cm⁻¹ and 20 000–500 cm⁻¹, respectively)

UV/VIS spectroscopy

Measurement of absorption in the 190–900 nm range (liquid and solid samples), temperature-dependent measurements with 6 cell changer

Cyclic voltammetry

Determination of redox potentials in organic solvents or aqueous solution

Potentiometry

Potentiometric pH titrations

Spectroelectrochemistry

Simultaneous determination of reduction/oxidation potentials and absorption in the UV/VIS/NIR range

AAS

Atomic absorption via flame, graphite furnace and hydride generation technique for the analysis of metals and metalloids

ICP-MS

Determination of elements with a double-focusing high-resolution mass spectrometer (except halogens, noble gases, C, N, H, O)

ICP-OES

Determination of elements via atomic and ionic emission (except halogens, noble gases, C, N, H, O)

Microwave for digestion reactions

acidic and basic digestions of solid substances

Acid distillation

Purification of acids using subboiling distillation

GC-MS

Separation and identification of (volatile) organic compounds by gas chromatography coupled with mass spectrometry

Gel electrophoresis

Separation and analysis of protein (fragments) (vertical, PAGE) and DNA (horizontal, agarose)

Gel scanner

Visualization und quantification of gel electrophoresis experiments with different trays depending on gel staining method

• UV Sample Tray (for irradiation with UV light)
• Stain-free Sample Tray (for stain-free gels)
• White Sample Tray (Coomassie Blue, Copper, Silver, Zinc staining)

HPLC

Purification (semi-preparative, max. 50 mg) and determination of purity (analytical) with autosampler of organic compounds, especially peptides

Incubation and mixing

Incubation of solution in Eppendorf tubes (0.5 and 1.5 mL) in a temperature range 1–100 °C at max. 3000 rpm, centrifugation and vortexing

Lyophilizer

Lyophilization (freeze drying) of aqueous samples

Synthesis

• Organic synthesis and coordination chemistry, partially under inert gas atmosphere
• Reactions in parallel synthesis reactor on a small scale (irradiation possible)
• Solid phase peptide synthesis and bioconjugate chemistry

Centrifuge

Centrifugation of  Falcon tubes (15 and 50 mL) and Eppendorf tubes (1,5 and 2 mL) in a temperature range of -9 to 40 °C at max. 4400 rpm