In many of our projects, we have to work with degraded DNA, either from museum specimens such as skins or skeleton or fossil bones and teeth. Therefore, we also work on technical developments of the individual steps in obtaining DNA sequences from the extraction of DNA to library construction and hybridization capture.
Recent selected publications
Pinhasi, R., Fernandes, D., Sirak, K., Novak, M., Connell, S., Alpaslan-Roodenberg, S., Gerritsen, F., Moiseyev, V., Gromov, A., Raczky, P., Anders, A., Pietrusewsky, M., Rollefson, G., Jovanovic, M., Trinhhoang, H., Bar-Oz, G., Oxenham, M., Matsumura, H. and Hofreiter, M. (2015) Optimal Ancient DNA Yields from the Inner Ear Part of the Human Petrous Bone. PLoS One, 10 (6), e0129102 (published online 18th June 2015, doi: 10.1371/journal.pone.0129102 eCollection 2015).
Paijmans, J.L.A., Fickel, J., Courtiol, A., Hofreiter, M. and Förster, D.W. (2015) Impact of enrichment conditions on cross-species capture of ancient, archival and fresh DNA. Molecular Ecology Resources, 16 (1), 42-55 (published online 27th April 2015, doi: 10.1111/1755-0998.12420).
Teasdale, M.D., van Doorn, N.L., Fiddyment, S., Webb, C., O'Connor, T., Hofreiter, M., Collins, M.J. and Bradley, D.G. (2015) Reading the leaves: parchment as a reservoir of ancient DNA for next generation sequencing. Phil Trans R Soc B, 370 (1660), 20130379 (doi: 10.1098/rstb.2013.0379).
Li, C., Hofreiter, M., Straube, N., Corrigan, S. and Naylor, G.J.P. (2013) Capturing protein-coding genes across highly divergent species. Biotechniques, 54, 321-326 (doi: 10.2144/000114039).
Heyn, P., Stenzel, U., Briggs, A. W., Kircher, M., Hofreiter, M. and Meyer, M. (2010) Uncovering the frequency of blocking lesions in ancient DNA. Nucleic Acids Research, 38 (16), e161 (published online 28th June 2010; doi:10.1093/nar/gkq572).