Latest Publications

Monya Baker, Editor, Nature Reports Stem Cells

„Data supplied by both users and companies can be sparse, and some projects share data only if they confirm that an antibody works as expected. “Sometimes it seems easier to hire a detective than to order a specific antibody,” concludes an overview of antibody portals“

 

 

Pleasant news:

Katja Hanacks and Diana Paulys research article "How to avoid pitfalls in antibody use" was cited by Monya Baker in her nature article "Antibodies used in research often give murky results. Broader awareness and advanced technologies promise clarity".

Monya Baker, Editor, Nature Reports Stem Cells

„Data supplied by both users and companies can be sparse, and some projects share data only if they confirm that an antibody works as expected. “Sometimes it seems easier to hire a detective than to order a specific antibody,” concludes an overview of antibody portals“


publications 2022

Hirschberg, S., Bauer H., Kamhieh-Milz, J., Ringel, F., Harms, C., Eddin, OK., Pruß, A., Hanack, K., Schulze-Forster, K. (2022).

SARS-CoV-2 Virus-like Particles (VLPs) Specifically Detect Humoral Immune Reactions in an ELISA-Based Platform.

Antibodies 2022, 11, 76. doi.org/10.3390/antib11040076

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Michelchen, S., Micheel, B., Hanack, K. (2022).

Antigenspezifische Aktivierung von B-Lymphozyten in vitro.

BIOspektrum. DOI: 10.1007/s12268-022-1829-z.

Schlör, A., Hirschberg, S., Amor, GB., Meister, TL., Arora, P., Pöhlmann, S., Hoffmann, M., Pfaender, S., Eddin, OK., Kamhieh-Milz, J., Hanack, K. (2022).

SARS-CoV-2 neutralizing camelid heavy-chain-only antibodies as powerful tools for diagnostic and therapeutic applications.

Front Immunol. 2022 Sep 14;13:930975. doi: 10.3389/fimmu.2022.930975.

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Gätjen, D., Wieczorek, M., Listek, M., Tomszak, F., Nölle, V., Hanack, K., Droste, M. (2022).

A switchable secrete-and-capture system enables efficient selection of Pichia pastoris clones producing high yields of Fab fragments.

Journal of Immunological Methods. doi.org/10.1016/j.jim.2022.113383.

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Chaudhari, A., Joshi, M., Kumar, D., Patel, A., Lokhande, K.,B., Krishnan, A., Hanack, K., Filipek, S., Liepmann, D., Renugopalakrishnan, V., Paulmurugan, R., Joshi, C. (2022).

Evaluation of Immune Evasion in SARS-CoV-2 Delta and Omicron variants.

Computational and Structural Biotechnology Journal. doi.org/10.1016/j.csbj.2022.08.010.

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Engel, R., Micheel, B., Hanack, K. (2022).

Three-dimensional cell culture approach for in vitro immunization and the production of monoclonal antibodies.

Biomedical Materials 2022 Jun 21. doi: 10.1088/1748-605X/ac7b00.

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Czarnecka, M., Weichelt, U., Rödiger, S., Hanack, K. (2022).

Novel Anti Double-Stranded Nucleic Acids Full-Length Recombinant Camelid Heavy-Chain Antibody for the Detection of miRNA.

Int. J. Mol. Sci. 2022, 23(11), 6275; doi.org/10.3390/ijms23116275.

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publications 2021

Fudickar, W., Roder, P., Listek, M., Hanack, K., Linker T. (2021).

Pyridinium Alkynylanthracenes as Sensitizers for Photodynamic Therapy.

Photochem Photobiol  2021 Oct 31. doi: 10.1111/php.13554.

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Göthel, M., Listek, M., Messerschmidt, K., Schlör, A., Hönow, A., Hanack, K. (2021).

A New Workflow to Generate Monoclonal Antibodies against Microorganisms.

Appl. Sci. 2021, 11(20), 9359; doi.org/10.3390/app11209359

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Michelchen, S., Micheel, B., Hanack, K. (2020).

In vitro immunization approach to generate specific murine monoclonal IgG antibodies.

bioRxiv. doi.org/10.1101/2020.11.24.393728

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publications 2020

Listek, M., Hönow, A.,  Gossen, M., Hanack, K. (2020).

A novel selection strategy for antibody producing hybridoma cells based on a new transgenic fusion cell line.

Sci Rep

10, 1664 (2020). doi.org/10.1038/s41598-020-58571-w

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publications 2019

Lütkecosmann, S., Faupel, T., Porstmann, S., Porstmann, T., Micheel, B., Hanack, K. (2019).

A cross-reactive monoclonal antibody as universal detection antibody in autoantibody diagnostic assays.

Clin Chim Acta. 2019 Sep 4;499:87-92. doi: 10.1016/j.cca.2019.09.003.

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publications 2018

Schlör, A., Holzlöhner, P., Listek, M., Grieß, C., Butze, M., Micheel, B., Hentschel, C., Sowa, M., Roggenbuck, D., Schierack, P., Füner, J., Schliebs, E., Goihl, A., Reinhold, D., Hanack, K. (2018).

Generation and validation of murine monoclonal and camelid recombinant single domain antibodies specific for human pancreatic glycoprotein 2.

N Biotechnol. 2018 Apr 7. pii: S1871-6784(17)30269-8. doi: 10.1016/j.nbt.2018.03.006.

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Holzlöhner, P., Butze, M., Maier, N., Hebel, N., Schliebs, E., Micheel, B., Füner, J., Heidicke, G., Hanack, K. (2018).

Generation of murine monoclonal antibodies with specificity against conventional camelid IgG1 and heavy-chain only IgG2/3

Veterinary Immunology and Immunopathology, Volume 197, March 2018, Pages 1–6


publications 2017

Wittig, BM., Sabat, R., Holzlöhner, P., Witte-Händel, E., Heilmann, K., Witte, K., ... & Günthert, U. (2017).

Absence of specific alternatively spliced exon of CD44 in macrophages prevents colitis.

Mucosal Immunol. Nov 29. doi: 10.1038/mi.2017.98. 

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Roggenbuck, D., Goihl, A., Hanack, K., Holzlöhner, P., Hentschel, C., Veiczi, M., Schierack, P., Reinhold, D., Schulz, HU. (2017).

Serological diagnosis and prognosis of severe acute pancreatitis by analysis of serum glycoprotein 2.

Clin Chem Lab Med. May 1;55(6):854-864., doi: 10.1515/cclm-2016-0797.

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 Holzlöhner, P., Hanack, K. (2017).

Generation of Murine Monoclonal Antibodies by Hybridoma Technology.

J. Vis. Exp. (119), e54832, doi:10.3791/5483

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publications 2016

Lütkecosmann, S., Warsinke, A., Tschöpe, W., Eichler, R., & Hanack, K. (2016).

A novel monoclonal antibody suitable for the detection of leukotriene B4.

Biochemical and Biophysical Research Communications.

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Roggenbuck, D., Goihl, A., Hanack, K., Holzlöhner, P., Hentschel, C., Veiczi, M., Schierack, P., Reinhold, D., Schulz, HU. (2016).

Serological diagnosis and prognosis of severe acute pancreatitis by analysis of serum glycoprotein 2.

Clinical Chemistry and Laboratory Medicine (CCLM).

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Hanack, K., Messerschmidt, K., & Listek, M. (2016).

Antibodies and Selection of Monoclonal Antibodies.

Protein Targeting Compounds (pp. 11-22). Springer International Publishing.

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Roggenbuck, D., Borghi, M. O., Somma, V., Büttner, T., Schierack, P., Hanack, K., ... & Boccellato, F. (2016).

Antiphospholipid antibodies detected by line immunoassay differentiate among patients with antiphospholipid syndrome, with infections and asymptomatic carriers.

Arthritis research & therapy, 18(1), 1.

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Schmidt, C., Rödiger, S., Gruner, M., Moncsek, A., Stohwasser, R., Hanack, K., Schierack, P., Schröder, C. (2016).

Multiplex localization of sequential peptide epitopes by use of a planar microbead chip.

Analytica Chimica Acta.

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publications 2015

Pauly, D., & Hanack, K. (2015).

How to avoid pitfalls in antibody use.

F1000Research.

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Kaba, H. E., Maier, N., Schliebe-Ohler, N., Mayer, Y., Müller, P. P., van den Heuvel, J., Schuchardt, J., Hanack, K., Bilitewski, U. (2015).

Identification of whole pathogenic cells by monoclonal antibodies generated against a specific peptide from an immunogenic cell wall protein.

Journal of microbiological methods, 108, 61-69.

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