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Mass Spectrometry

Mass Spectrometry

The microbiology group offers services in mass spectrometry, our expertise lies in the analytics of small peptides, especially in targeted and non-targeted metabolomics of small molecules (also known as “natural products”). We also offer basic proteomic analyses. For all our analyses we employ an Orbitrap Exploris 240 ESI-LC-MS system.

For further information and to discuss your project individually, please contact one of our staff members.

 

Responsible persons

 

Peptide analytics

Dr. María Miguel 

Tel.: +49 331 977-290057

E-Mail: maria.miguel@uni-potsdam.de

Lena Uhlig

Tel.: +49 331 977 6131

E-Mail: uhlig2@uni-potsdam.de

 

Proteomics

Dr. Arthur Guljamow 

Tel.: +49 331 977-290057

E-Mail: guljamow@uni-potsdam.de

 

Instrumentation

UHPLC (Thermo Vanquish System) coupled to a Hybrid Quadrupole-Orbitrap HRMS (Thermo Orbitrap Exploris 240)

Operated Column:

  • Phenomenex Kinetex® 1.7µm C18 100Å

50 x 2.1 mm

with guard column C18

 

ESI (Heated Electrospray Ionization) source

MS and MS/MS (HCD fragmentation)

Resolution up to 240,000 FWHM (at m/z 200)

Mass range: m/z 40-6000

 

Sample submission

Please submit samples in a 2 mL autosampler vial with an inlet. Inlets must contain a minimum of 150 µL of sample and must be labeled with name, date, solvent and sample name.