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Intrinsically Disordered Proteins - Publications

Please also refer to: pubmed.ncbi.nlm.nih.gov

Articles in peer-reviewed journals

  1. Rindfleisch T, Nencini R, Ollila OHS, Walther D, Miettinen MS*, Thalhammer A*. Molecular Dynamics of the Intrinsically Disordered Protein COR15A-A Force Field Validation on Structure and Dynamics, accepted
  2. Marks RA, Ekwealor JTB, Artur MAS, Bondi L, Boothby TC, Carmo OMS, Centeno DC, Coe KK, Dace HJW, Field S, Hutt A, Porembski S, Thalhammer A, van der Pas L, Wood AJ, Alpert P, Bartels D, Boeynaems S, Datar MN, Giese T, Seidou WI, Kirchner SM, Köhler J, Kumara UGVSS, Kyung J, Lyall R, Mishler BD, Ndongmo JBVT, Otegui MS, Reddy V, Rexroth J, Tebele SM, VanBuren R, Verdier J, Vothknecht UC, Wittenberg MF, Zokov E, Oliver MJ, Rhee SY. Life on the dry side: a roadmap to understanding desiccation tolerance and accelerating translational applications. Nat Commun. 2025;16(1):3284.
  3. Wojciechowska I, Mukherjee T, Knox-Brown P, Hu X, Khosla A, Subedi B, Ahmad B, Mathews GL, Panagakis AA, Thompson KA, Peery ST, Szlachetko J, Thalhammer A, Hincha DK, Skirycz A, Schrick K. Arabidopsis PROTODERMAL FACTOR2 binds lysophosphatidylcholines and transcriptionally regulates phospholipid metabolism. New Phytol. 2024;244(4):1498-1518.
  4. Skoulas D, Ojo OM, Thalhammer A, Kochovski Z, Schlaad H. Solution Behavior of Glyco-Copoly(l-Glutamic Acid)s in Dilute Saline Solution. Biomacromolecules. 2024;25(6):3724-3730.
  5. Hernández-Sánchez I, Rindfleisch T, Alpers J, Dulle M, Garvey CJ, Knox-Brown P, Miettinen MS, Nagy G, Pusterla JM, Rekas A, Shou K, Stadler AM, Walther D, Wolff M, Zuther E, Thalhammer A. Functional in vitro diversity of an intrinsically disordered plant protein during freeze-thawing is encoded by its structural plasticity. Protein Sci. 2024;33(5):e4989.
  6. Uflewski M, Rindfleisch T, Korkmaz K, Tietz E, Mielke S, Correa Galvis V, Dünschede B, Luzarowski M, Skirycz A, Schwarzländer M, Strand DD, Hertle AP, Schünemann D, Walther D, Thalhammer A, Wolff M, Armbruster U. The thylakoid proton antiporter KEA3 regulates photosynthesis in response to the chloroplast energy status. Nat Commun. 2024;15(1):2792.
  7. Hernández-Sánchez IE, Maruri-López I, Martinez-Martinez C, Janis B, Jiménez-Bremont JF, Covarrubias AA, Menze MA, Graether SP, Thalhammer A. LEAfing through literature: late embryogenesis abundant proteins coming of age-achievements and perspectives. J Exp Bot. 2022;73(19):6525-6546.
  8. Wolff M, Gast K, Evers A, Kurz M, Pfeiffer-Marek S, Schüler A, Seckler R, Thalhammer A. A conserved hydrophobic moiety and helix–helix interactions drive the self-assembly of the incretin analog Exendin-4. Biomolecules. 2021; 11(9):1305
  9. Knox-Brown P, Rindfleisch T, Günther A, Balow K, Bremer A, Walther D, Miettinen MS, Hincha DK, Thalhammer A. Similar yet different-structural and functional diversity among Arabidopsis thaliana LEA_4 proteins. Int J Mol Sci. 2020;21(8):2794.
  10. Wolff M, Schüler A, Gast K, Seckler R, Evers A, Pfeiffer-Marek S, Kurz M, Nagel N, Haack T, Wagner M, Thalhammer A. Self-assembly of Exendin-4-derived dual peptide agonists is mediated by acylation and correlated to the length of conjugated fatty acyl chains. Mol Pharm. 2020;17(3):965-978.
  11. Shou K, Bremer A, Rindfleisch T, Knox-Brown P, Hirai M, Rekas A, Garvey CJ, Hincha DK, Stadler AM*, Thalhammer A*. Conformational selection of the intrinsically disordered plant stress protein COR15A in response to solution osmolarity - an X-ray and light scattering study. Phys Chem Chem Phys. 2019;21(34):18727-18740
  12. Sowemimo OT, Knox-Brown P, Borcherds W, Rindfleisch T, Thalhammer A*, Daughdrill GW*. Conserved Glycines Control Disorder and Function in the Cold-Regulated Protein, COR15A. Biomolecules. 2019;9(3). pii: E84
  13. Navarro-Retamal C, Bremer A, Ingólfsson HI, Alzate-Morales J, Caballero J, Thalhammer A, González W, Hincha DK. Folding and lipid composition determine membrane interaction of the disordered protein COR15A. Biophys J. 2018;115(6):968-980
  14. Broeker NK, Kiele F, Casjens SR, Gilcrease EB, Thalhammer A, Koetz J, Barbirz S. In Vitro Studies of Lipopolysaccharide-Mediated DNA Release of Podovirus HK620. Viruses. 2018 May 29;10(6).
  15. Sprenger H, Erban A, Seddig S, Rudack K, Thalhammer A, Le MQ, Walther D, Zuther E, Köhl KI, Kopka J, Hincha DK. Metabolite and transcript markers for the prediction of potato drought tolerance. Plant Biotechnol J. 2018;16(4):939-950
  16. Gast K, Schüler A, Wolff M, Thalhammer A, Berchtold H, Nagel N, Lenherr G, Hauck G, Seckler R. Rapid-acting and human insulins: Hexamer dissociation kinetics upon dilution of the pharmaceutical formulation. Pharm Res. 2017;34(11):2270-2286
  17. Bremer A, Kent B, Hauß T, Thalhammer A, Yepuri NR, Darwish TA, Garvey CJ, Bryant G, Hincha DK. Intrinsically disordered stress protein COR15A resides at the membrane surface during dehydration. Biophys J. 2017;113(3):572-579
  18. Braig F, Kriegs M, Voigtlaender M, Habel B, Grob T, Biskup K, Blanchard V, Sack M, Thalhammer A, Ben Batalla I, Braren I, Laban S, Danielczyk A, Goletz S, Jakubowicz E, Märkl B, Trepel M, Knecht R, Riecken K, Fehse B, Loges S, Bokemeyer C, Binder M. Cetuximab resistance in head and neck cancer is mediated by EGFR-K(521) polymorphism. Cancer Res. 2017;77(5):1188-1199.
  19. Bremer A, Wolff M, Thalhammer A, Hincha DK. Folding of intrinsically disordered plant LEA proteins is driven by glycerol-induced crowding and the presence of membranes. FEBS J. 2017;284(6):919-936
  20. Navarro-Retamal C, Bremer A, Alzate-Morales J, Caballero J, Hincha DK, González W, Thalhammer A. Molecular dynamics simulations and CD spectroscopy reveal hydration-induced unfolding of the intrinsically disordered LEA proteins COR15A and COR15B from Arabidopsis thaliana. Phys Chem Chem Phys. 2016;18(37):25806-16
  21. Thalhammer A, Hincha DK. A mechanistic model of COR15 protein function in plant freezing tolerance: integration of structural and functional characteristics. Plant Signal Behav. 2014;9(12):e977722
  22. Thalhammer A, Bryant G, Sulpice R, Hincha DK. Disordered cold regulated15 proteins protect chloroplast membranes during freezing through binding and folding, but do not stabilize chloroplast enzymes in vivo. Plant Physiol. 2014;166(1):190-201
  23. Hincha DK, Thalhammer A. LEA proteins: IDPs with versatile functions in cellular dehydration tolerance. Biochem Soc Trans. 2012;40(5):1000-3. Review.
  24. Thalhammer A, Hundertmark M, Popova AV, Seckler R, Hincha DK. Interaction of two intrinsically disordered plant stress proteins (COR15A and COR15B) with lipid membranes in the dry state. Biochim Biophys Acta. 2010;1798(9):1812-20. 

Book chapters

  1. Thalhammer A, Bröker NK. Biophysical Approaches for the Characterization of Protein-Metabolite Interactions. Methods Mol Biol. 2023;2554:199-229.
  2. Thalhammer A, Pagter M, Hincha DK, Zuther E. Measuring freezing tolerance of leaves and rosettes: Electrolyte leakage and chlorophyll fluorescence assays. Methods Mol Biol. 2020;2156:9-21.
  3. Stuchfield D, France AP, Migas LG, Thalhammer A, Bremer A, Bellina B, Barran PE. The Use of Mass Spectrometry to Examine IDPs: Unique Insights and Caveats. Methods Enzymol. 2018;611:459-50
  4. Thalhammer A, Hincha DK, Zuther E. Measuring freezing tolerance: electrolyte leakage and chlorophyll fluorescence assays. Methods Mol Biol. 2014;1166:15-24
  5. Thalhammer A, Hincha DK.  Plant and microbe adaptations to cold in a changing world. New York: Springer; 2013. Chapter 8, The Function and Evolution of Closely Related COR/LEA (Cold-Regulated/Late Embryogenesis Abundant) Proteins in Arabidopsis thaliana; p. 89-105

Preprints

  1. Eldrid C, Stuchfield D, Bath Alén A, Thalhammer A, Barran P. Mapping the Conformational Landscape of the Cold-Regulated Intrinsically Disordered Protein COR15A. ChemRxiv. 2025; doi:10.26434/chemrxiv-2025-58ft6
  2. Rindfleisch T, Nencini R, Ollila OHS, Walther D, Miettinen MS*, Thalhammer A*. Molecular dynamics of the intrinsically disordered protein COR15A — A force field validation on structure and dynamics. ChemRxiv. 2025; doi:10.26434/chemrxiv-2025-zqh59
  3. Uflewski M, Rindfleisch T, Korkmaz K, Tietz E, Mielke S, Correa Galvis V, Dünschede B, Luzarowski M, Skirycz A, Schwarzländer M, Strand DD, Hertle AP, Schünemann D, Walther D, Thalhammer A, Wolff M, Armbruster U. The K+ exchange antiporter 3 senses the chloroplast energy status to synchronize photosynthesis. bioRxiv 2022.12.19.521033
  4. Wojciechowska I, Mukherjee T, Knox-Brown P, Hu X, Khosla A, Mathews GL, Thompson KA, Peery ST, Szlachetko J, Thalhammer A, Hincha DK, Skirycz A, Schrick K. Arabidopsis PROTODERMAL FACTOR2 binds lysophosphatidylcholines and transcriptionally regulates phospholipid metabolism. bioRxiv 2021.10.20.465175

* marks shared crresponding authorships