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Original publications of the work group

Odell J., Gräf, R., and Lammerding, J. (2023) Heterologous expression of Dictyostelium discoideum NE81 in mouse embryo fibroblasts reveals conserved mechanoprotective roles of lamins. Mol. Biol. Cell. doi: 10.1091/mbc.E23-05-0193. Online ahead of print

Mitic, K., Meyer, I., Gräf, R., and Grafe, M. (2023) Temporal changes of nuclear envelope permeability during semi-closed mitosis in Dictyostelium amoebae. Cells, 12(10), 1380,

Schweigel, U., Batsios, P., Müller-Taubenberger, A., Gräf, R., and Grafe, M. (2022) Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis. Nucleus, 13, 144-154,

Mitic, K., Grafe, M., Batsios, P. , and Meyer, I. (2022) Partial Disassembly of the Nuclear Pore Complex Proteins during Semi-Closed Mitosis in Dictyostelium discoideum. Cells, 11(3), 407;

Pitzen, V., Sander, S., Baumann, O., Gräf, R., and Meyer, I. (2021) Cep192, a Novel Missing Link between the Centrosomal Core and Corona in Dictyostelium Amoebae. Cells, 10, 2384.

Grafe, M., Hofmann, P., Batsios, P., Meyer, I., and Gräf, R. (2020) In vivo assembly of a Dictyostelium lamin mutant induced by light, mechanical stress, and pH. Cells, 9, (8), 1834;

Koonce, M., Tikhonenko, I., and Gräf, R. (2020) Dictyostelium Cell Fixation: Two Simple Tricks. Methods Protoc., 3(3), 47;

Neukranz, Y., Kotter, A., Beilschmidt, L., Marelja, Z., Helm, M., Gräf, R., and Leimkühler, S. (2019) Analysis of the cellular roles of MOCS3 identifies a MOCS3-independent localization of NFS1 at the tips of the centrosome. Biochemistry 58, 1786-1798

Batsios P., Ishikawa-Ankerhold H.C., Roth H., Schleicher M., Wong C.C.L., Müller-Taubenberger A. (2019) Ate1-Mediated Posttranslational Arginylation Affects Substrate Adhesion and Cell Migration in Dictyostelium discoideum. Mol Biol Cell. 30, 453-466

Grafe, M., Batsios, P., Meyer, I., Lisin, D., Baumann, O., Goldberg, M.W. and Gräf, R. (2019) Supramolecular Structures of the Dictyostelium Lamin NE81.Cells, 8, 162; doi:10.3390/cells8020162

Mai, T., Wolski, K., Puciul-Malinowska, A., Kopyshev, A., Gräf, R., Bruns, M., Zapotoczny, S. and Taubert, A. (2018) Anionic Polymer Brushes for Biomimetic Calcium Phosphate Mineralization - A Surface with Application Potential in Biomaterials. Polymers, 10 (10), 1165

Pitzen, V., Askarzada, S., Gräf, R., Meyer, I. (2018) CDK5RAP2 is an essential scaffolding protein of the corona of the Dictyostelium centrosome. Cells, 7, 04, doi:10.3390/cells7040032

Meyer, I., Peter, T., Batsios, P., Kuhnert, O., Krüger-Genge, A., Camurça, C. and Gräf, R. (2017) CP39, CP75 and CP91 are major structural components of the Dictyostelium centrosome's core structure. Eur. J. Cell Biol., 96, 119-130

Hartmann, B., Wai, T., Hu, H., Musante, L., Fischer-Zirnsak, B., Stenzel, W., Gräf, R., van den Heuvel, L., Ropers, H.H., Wienker, T.F., Hübner, C., Langer, T. and Kaindl, A.M. (2016) Homozygous YME1L1 Mutations Cause Mitochondriopathy with Optic Atrophy via OPA1- dependent Mitochondrial Network Fragmentation. eLIFE, 5, DOI 10.7554/eLife.16078

Batsios, P., Ren, X., Baumann, O., Larochelle, D.A. and Gräf, R. (2016) Src1 is a protein of the inner nuclear membrane interacting with the Dictyostelium lamin NE81. Cells, 5, (1), pii: E13. doi: 10.3390/cells5010013

Putzler, S., Meyer, I., Gräf, R. (2016) CP91 is a component of the Dictyostelium centrosome involved in centrosome biogenesis. Eur. J. Cell Biol., 95, 124-135.

Batsios, P., Meyer, I., Gräf, R. (2016) Proximity-dependent biotin identification (BioID) in Dictyostelium amoebae. Methods Enzymol. 569, 23-43.

Kraemer, N., Ravindran, E., Zaqout, S., Neubert, G., Schindler, D., Ninnemann, O., Gräf, R., Seiler, A.E.M., and Kaindl, A.M. (2015) Loss of CDK5RAP2 affects neural but not non-neuronal mESC differentiation into cardiomyocytes. Cell Cycle, 14, 2044-2057

Junemann, A., Winterhoff, M., Nordholz, B., Rottner, K., Eichinger, L., Gräf, R., and Faix, J. (2013) ForC lacks canonical formin activity but bundles actin filaments and is required and multicellular development of Dictyostelium cells. Eur. J. Cell Biol. 92, 201-212

Müller, S., Windhof, I., Maximov, V., Jurkowski, T.P., Jeltsch, A., Förstner, K., Sharma, C., Gräf, R., and Nellen, W. (2013) Target recognition, RNA methylation activity and transcriptional regulation of the Dictyostelium discoideum Dnmt2-homologue (DnmA). Nucl. Acids Res. 41, 8615-8627

Tikhonenko, I., Magidson, V., Gräf, R., Khodjakov, A., and Koonce, M. P. (2013). A kinesin-mediated mechanism that couples centrosomes to nuclei. Cell. Mol. Life Sci. 70, 1285-1296

Kuhnert, O., Baumann, O., Meyer, I. and Gräf, R. (2012). CP55, a novel key component of centrosomal organization in Dictyostelium. Cell Mol Life Sci 69, 3651-3664

Kuhnert, O., Baumann, O., Meyer, I. and Gräf, R. (2012). Functional characterization of CP148, a novel key component for centrosome integrity in Dictyostelium. Cell Mol Life Sci 69, 1875-1888.

Krüger, A., Batsios, P., Baumann, O., Luckert, E., Schwarz, H., Stick, R., Meyer, I. and Gräf, R. (2012). Characterization of NE81, the first lamin-like nucleoskeleton protein in a unicellular organism. Mol. Biol. Cell 23, 360-370.

Samereier, M., Baumann, O., Meyer, I. and Gräf, R. (2011) Analysis of Dictyostelium TACC reveals differential interactions with CP224 and unusual dynamics of Dictyostelium microtubules. Cell. Mol. Life Sci. 68, 275-287

Samereier, M., Meyer, I., Koonce, M.P., and Gräf, R. (2010) Live cell imagine techniques for analyses of microtubules in Dictyostelium. Methods in Cell Biology 97, 341-357

Dubin, M., Fuchs, J., Gräf, R., Schubert, I. and Nellen, W. (2010) Dynamics of a novel centromeric histone variant CenH3 reveals the evolutionary ancestral timing of centromere biogenesis. Nucleic Acids Res. 38, 7526-7537

Schulz, I., Baumann, O., Samereier, M., Zoglmeier, C., and Gräf, R. (2009) Dictyostelium Sun1 is a dynamic membrane protein of both nuclear membranes and required for centrosomal association with clustered centromers. Eur. J. Cell Biol. 11, 621-638

Mana-Capelli, S., Gräf, R., and Larochelle, D.A. (2009) Dictyostelium discoideum CenB is a bona fide centrin essential for nuclear architecture and centrosome stability. Eukaryotic Cell 8, 1106-1117

Schulz, I., Erle, A., Gräf, R., Krüger, A., Lohmeier, H., Putzler, S., Samereier, M., and Weidenthaler, S. (2009) Identification and cell cycle-dependent localization of nine novel, genuine centrosomal components in Dictyostelium discoideum. Cell Motil. Cytoskeleton, 66, 915-928

Shkilnyy, A., Gräf, R., Hiebl, B., Neffe, A.T., Friedrich, A., Hartmann, J., and Taubert, A. (2009) Unprecedented, low cytotoxicity of spongelike calcium phosphate/poly(ethylene imine) hydrogel composites. Macromol Biosci. 9, 179-86

Li, H., Chen, Q., Kaller, M., Nellen, W., Gräf, R., and De Lozanne, A. (2008) Dictyostelium Aurora kinase has properties of both Aurora A and Aurora B kinases. Eukaryotic Cell 7, 894-905

Sharma, S., Kulk, N., Nold,, M. F., Gräf, R., Kim, S.-H., Reinhardt, D., Dinarello, C. A. and Bufler, P. (2008) The interleukin1 family member 7b translocates to the nucleus and downregulates proinflammatory cytokines. J. Immunol. 180, 5477-5482

Koch, K. V., Reinders, Y, Ho, T.-H., Sickmann, A. and Gräf R. (2006) Identification and isolation of Dictyostelium microtubule-associated protein interactors by tandem affinity purification. Eur. J. Cell Biol. 85, 1079 - 1090

Reinders, Y., Schulz, I., Gräf, R. and Sickmann, A. (2006) Identification of novel centrosomal proteins in D. discoideum by comparative proteomic approaches. J. Proteome Res. 5, 589 - 598

Rehberg, M., Kleylein-Sohn, J., Faix, J., Ho, T.-H., Schulz, I., and Gräf, R. (2005) Dictyostelium LIS1 is a centrosomal protein required for microtubule/cell cortex interactions, nucleus/centrosome linkage and actin dynamics. Mol. Biol. Cell 16, 2759-2771

Hestermann, A. and Gräf, R. (2004) The XMAP215-family protein DdCP224 is required for cortical interactions of microtubules. BMC Cell Biol. 5, 24

Gräf, R., Euteneuer, U., Ho, Thi-Hieu and Rehberg, M. (2003) Regulated expression of the centrosomal protein DdCP224 affects microtubule dynamics and reveals mechanisms for the control of supernumerary centrosome number, Mol. Biol. Cell 14, 4067 - 4074.

Rehberg, M. and Gräf, R. (2002) Dictyostelium EB1 is a centrosomal component required for proper spindle formation. Mol. Biol. Cell 13, 2301-2310.

Gräf, R. (2002) DdNek2, the first non-vertebrate homologue of human Nek2, is required for centrosome duplication in Dictyostelium. J. Cell Sci.115, 1919-1929.

Daunderer, C. and Gräf, R. (2002) Molecular analysis of the cytosolic Dictyostelium ?-tubulin complex. Eur. J. Cell Biol. 81, 175-184.

Gräf, R. (2001) Maltose-binding protein as a fusion tag for the localization and purification of cloned proteins in Dictyostelium. Anal. Biochem., 289, 297 - 300.

Daunderer, C., Schliwa, M. and Gräf, R. (2001) Dictyostelium centrin-related protein (DdCrp), the most divergent member of the centrin family, possesses only two EF-hands and dissociates from the centrosome during mitosis. Eur. J. Cell Biol. 80, 621-630.

Panteris, E., Apostolakos, P., Gräf, R. and Galatis, B. (2000) gamma-Tubulin colocalizes with microtubule arrays and tubulin paracrystals in dividing vegetative cells of higher plants. Protoplasma. 210, 179 - 187.

Gräf, R., Daunderer, C. and Schliwa, M. (2000) Dictyostelium DdCP224 is a microtubule-associated protein and a permanent centrosomal resident involved in centrosome duplication. J. Cell Sci. 113, 1747 – 1758.

Ma, S., Triviños-Lagos, L., Gräf, R. and Chisholm, R.L. (1999) Dynein Intermediate Chain Mediated Dynein-Dynactin Interaction is Required for Interphase Microtubule Organization and Centrosome Replication and Separation in Dictyostelium. J. Cell Biol., 147, 1261 - 1273.

Gräf, R., Daunderer, C. and Schliwa, M. (1999). Cell cycle-dependent localization of monoclonal antibodies raised against isolated Dictyostelium centrosomes. Biol. Cell. 91, 471-477.

Gräf, R., Euteneuer, U., Ueda, M. and Schliwa, M. (1998). Isolation of nucleation-competent centrosomes from Dictyostelium discoideum. Eur. J. Cell. Biol. 76, 167 - 175.

Euteneuer, U., Gräf, R., Kube-Granderath, E. and Schliwa, M. (1998). Dictyostelium gamma-tubulin: molecular characterization and ultrastructural localization. J. Cell Sci. 111., 405 - 412

Ueda M., Gräf R., MacWilliams H. K., Schliwa M., Euteneuer U. (1997) Centrosome positioning and directionality of cell movements. Proc. Natl. Acad. Sci. USA 94, 9674 - 9678

Gräf R., Harvey W. R., Wieczorek H. (1996) Purification and properties of a cytosolic V1-ATPase. J. Biol. Chem. 271, 20908 - 20913

Lepier A., Gräf R., Azuma M., Merzendorfer H., Harvey W. R. and Wieczorek H. (1996) The peripheral complex of the tobacco hornworm V-ATPase contains a novel 13-kDa subunit G. J. Biol. Chem. 271, 8502 - 8508

Gräf R., Lepier A., Harvey W. R. and Wieczorek H. (1994). A Novel 14-kDa V-ATPase Subunit in the Tobacco Hornworm Midgut. J. Biol. Chem. 269, 3767 - 3774

Gräf R., Harvey W. R. and Wieczorek H. (1994). Cloning, sequencing and expression of cDNA encoding an insect V-ATPase subunit E. Biochim. Biophys. Acta 1190, 193 - 196

Novak F. J. S., Gräf R., Waring R. B., Wolfersberger M. G., Wieczorek H. and Harvey W. R. (1992). Primary structure of V-ATPase subunit B from Manduca sexta midgut. Biochim. Biophys. Acta 1132, 67 - 71

Gräf R., Novak F. J. S., Harvey W. R. and Wieczorek H. (1992). Cloning and sequencing of cDNA encoding the putative insect plasma membrane V-ATPase subunit A. FEBS Lett. 300, 119 - 122


Gräf, R., Grafe, M., Meyer, I., Mitic, K., and Pitzen, V. (2021) The Dictyostelium Centrosome. Cells, 10(10), 2657,

Batsios, P., Gräf, R., Koonce, M.P., Larochelle D.A., and Meyer I. (2019) Nuclear envelope organization in Dictyostelium discoideum. Int. J. Dev. Biol., 63, 509-519

Gräf, R. (2018) Comparative Biology of Centrosomal Structures in Eukaryotes. Cells, 11, 7, doi:10.3390/cells7110202

Gräf, R., Batsios, P., and Meyer, I. (2015) Evolution of centrosomes and the nuclear lamina: Amoebozoan assets. Eur. J. Cell Biol., 94, 249-256.

Gräf, R. (2014) Microtubule Organization in Dictyostelium (version 2.0). Encyclopedia of Life Sciences, DOI: 10.1002/9780470015902.a0021852.pub2

Devos, D.P., Gräf, R., and Field, M.C. (2014) Evolution of the nucleus. Curr. Opin. Cell Biol. 28C, 8-15

Batsios, P., Baumann, O., Meyer, I., and Gräf, R., (2012) Isolation of Dictyostelium nuclei for light and electron microscopy. Methods Mol. Biol., 983, 283-294.

Batsios, P., Peter, T., Baumann, O., Stick, R., Meyer, I., and Gräf, R., (2012) A lamin in lower eukaryotes? Nucleus, 3, 237-243

Meyer, I., Kuhnert, O., and Gräf, R. (2011). Functional analyses of lissencephaly-related proteins in Dictyostelium. Semin. Cell Dev. Biol. 22, 89-96.

Müller-Taubenberger, A. and Gräf, R. (2010) Centrosomen und Zytokinese bei Dictyostelium discoideum. Biospektrum, 04.10, 418-422

Mana-Capelli, S., Gräf, R., and Larochelle, D.A. (2010) Dictyostelium centrin B localization during cell cycle progression. Commun. Integr. Biol. 3, 39-41

Koonce, M.P., Gräf, R. (2010) Dictyostelium discoideum: A model system for ultrastructural analyses of cell motility and development. Methods in Cell Biology.

Gräf, R. (2009) Microtubule organization in Dictyostelium. In: Encyclopedia of Life Sciences (ELS), John Wiley and Sons Ltd., Chichester, DOI: 10.1002/9780470015902.a0001342.pub2.

Williams, R. S. B., Boeckeler, K. Gräf, R., Müller-Taubenberger, A., Li, Z., Isberg, R. R., Wessels, D., Soll, D. R., Alexander, H., and Alexander, S. (2006) Towards a molecular understanding of human diseases using Dictyostelium discoideum. Trends Mol. Med. 12, 415-424

Schulz, I., Wagner, Y., Sickmann, A. and Gräf R. (2006) An improved method for Dictyostelium centrosome isolation. Methods Mol. Biol. 346, 479 - 489

Gräf, R. and Wehland, J. (2005) Cytoskeletal dynamics; 6th Young Scientists Meeting of the German Society for Cell Biology (DGZ). Eur. J. Cell Biol. 84, 523-528

Gräf, R., Rietdorf, J. and Zimmermann, T. (2005) Live cell spinning disk microscopy. Adv. Biochem. Engin./Biotechnol. 95, 57 – 75

Gräf, R., Daunderer, C. and Schulz, I. (2004) Molecular and functional analysis of the Dictyostelium centrosome. Int. Rev. Cytol. 241, 155 - 202

Gräf, R., Herrmann-Lerdon, H. and Noegel, A. A. (2003) Nuclear Architecture; 5th Junior Scientist Conference of the German Society for Cell Biology (DGZ). Eur. J. Cell Biol. 82, 583-587

Hestermann, A., Rehberg, M. and Gräf, R. (2002). Centrosomal microtubule plus end tracking proteins and their role in Dictyostelium cell dynamics. J. Muscle Res. Cell Motil. 23, 621-630.

Gräf, R. (2001) Isolation of centrosomes from Dictyostelium. Methods Cell Biol. 67, 337-357.

Gräf, R., Brusis, N., Daunderer, C., Euteneuer, U., Hestermann, A., Schliwa, M. and Ueda, M. (2000). Comparative structural, molecular and functional aspects of the Dictyostelium discoideum centrosome. Curr. Top. Dev. Biol. 49, 161 - 185.

Daunderer, C., Schliwa, M. and Gräf, R. (1999). Dictyostelium discoideum: a promising centrosome model system. Biol. Cell. 91, 313-320.

Schliwa, M., Euteneuer, U., Gräf, R. and Ueda M. (1999). Centrosomes, microtubules, and cell migration. In Cell behavior: Control and Mechanism of Motility. J. M. Lackie, G. A. Dunn and G. E. Jones, editors. Portland Press, London. 223 - 231.

Merzendorfer H., Gräf, R., Huss M., Harvey W. R. and Wieczorek H. (1997) Regulation of proton-translocating V-ATPases. J. Exp. Biol. 200, 225 - 235