Zeiss LSM 780

The Zeiss LSM 780 is an inverted confocal laser scanning microscope for spectral selective fluorescence imaging. The system is equipped with a sensitive 32 channel array GaAsP detector flanked by two conventional multialkali PMTs allowing for simultaneous acquisition of multicolor samples and spectral unmixing.

• Software
  • Basic Software: Zen 2.1
  • Additional licences:
    • TILE
• Light Sources and Lasers:
  • Halogen lamp for transmitted light
  • External fluorescence lamp HXP 120 V
  • Lasers:
    • Argon Laser: 445 nm, 488 nm, 514 nm,
    • solid state diode laser: 405 nm,  561 nm, 633 nm
• Scanner:
  • Confocal point-scanning - optical sectioning
  • Zoom function
  • Scan field rotation
• Detectors
  • Spectral detector (GaAsP-PMT, 32 channels)
  • Two multialkali photonmultipliertubes (PMTs)
  • Transmission light detector (T-PMT)
• Objectives:
  • EC Plan-Neofluar 10x/0.3/Air M27
  • Plan-Apochromat 20x/0.8/Air M27
  • EC Plan-Neofluar 40x/1.3/Oil DIC M27
  • LD C Apochromat 40x /1.1/ Water Korr M27
  • C Apochromat 40x /1.2/Water Korr M27
  • Plan-Apochromat 63x /1.4/Oil M27
• Filters for Epi-Fluorescence
  • “DAPI” – Excitation Filter: G365, Dichroic: FT395, Emission Filter: BP 445/50
  • “GFP” - Excitation Filter: BP 470/40, Dichroic: FT 510, Emission Filter: BP 515-565
  • “Rhodamine” – Excitation Filter: BP 546/12, Dichroic: FT 560, Emission Filter: BP 575-640
• Dual band Emission filters for CLSM
  • VIS
    • MBS 458
    • MBS 458/514
    • MBS 458/561
    • MBS 488
    • MBS 488/561
    • MBS 488/633
    • MBS 488/561/633
  • UV
    • f-MBS 405/490c/640c
    • f-MBS 405/505c
    • f-MBS 405/520c
    • f-MBS 405/535c
    • f-MBS 405/550c
    • f-MBS 405/565c
    • f-MBS 405/580c
    • f-MBS 405/595c
    • f-MBS 405/610c
    • f-MBS 405/625c
• other specifications:
  • autofocus (definite focus),
  • motorized xy-stage
  • analyser module Pol ACR filter
  • Anisotropie P & S filter