Design and construction of complex DNA assembly projects via AssemblX

Graphic Assemblx Toolkit
Source: private
  • User-friendly
  • Webtool-based planning
  • Multi-gene assembly of 25 functional units, from 75 or more subunits
  • Scar-free, overlap-based and sequence-independent methods
  • Standardized, highly efficient, polymerase chain reaction-free and virtually sequence-independent scheme

 

https://assemblx.mpimp-golm.mpg.de/

Graphic Assemblx Toolkit
Source: private

Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, Mueller-Roeber B. (2017) AssemblX: a user-friendly toolkit for rapid and reliable multi-gene assemblies. Nucl. Acids Res. 6: 1742-1756.  PMID: 28130422.

Machens F., Hochrein L. (2020) The AssemblX Toolkit for Reliable and User-Friendly Multigene Assemblies. In: Chandran S., George K. (eds) DNA Cloning and Assembly. Methods in Molecular Biology, vol 2205. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0908-8_3